260 Breast Cancer Biopsy Triage with Ex-Vivo Microscopy for Downstream Analysis

OBJECTIVES/GOALS: In this study, the ability of a pathologist to detect malignancy on digital pseudo-H&E slides obtained via structured illumination microscopy (SIM) imaging fresh diagnostic breast biopsies was assessed. The speed and processing also assessed for potential clinical implementation. METHODS/STUDY POPULATION: This study conducted in accordance with an Ochsner Medical Center New Orleans IRB. 200 patients undergoing either stereotactic or ultrasound-guided were consented additional core from suspicious lesion collected research use. Research transported lab stained DRAQ5 Eosin-Y imaged SIM before being submitted histology processing. Imaging times recorded. resulting images given blind review assess accuracy. RESULTS/ANTICIPATED RESULTS: ex-vivo subsequent 79 cores have been date. Some samples excluded total data set not included final assessment due technical failures protocol. Of current set, has specificity 88% sensitivity 65%, as well NPV PPV 65%. Staining time each biopsy completed within 3 half minutes at 20x magnification took between 4 12 minutes, depending size implementation autofocus system. Image approximately 5 per is direct function size. DISCUSSION/SIGNIFICANCE: Decreased cancer suspicion treatment will improve prognosis patients. could ultimately allow primary secondary be performed simultaneously minimize histopathology time, thus allowing clinicians implement course more quickly.